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SIMS & NanoSIMS

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  • ACTINIS
    ACTINIS, Dynamic SIMS for analysis of highly radioactive samples

    Monday, December 14, 2020

    In this presentation recorded at the 2020 NuMat conference, Paula Peres introduces the ACTINIS Secondary Ion Mass Spectrometer: reminder of the basic principles of the SIMS technique, details of the shielding package providing all necessary protections against contamination and radiation effects while analyzing nuclear samples in ACTINIS, overview of the main applications.

    Duration : 20 minutes

  • NS Biology webinars
    Biological applications of NanoSIMS 50L: using isotopic tracer for studying cell origin, aging and renewal

    Tuesday, November 24, 2020

    Part 4 of our biology webinar series is illustrating the potential of labeling DNA with 15N stable isotope in order to study cell origin, age and renewal at single cell level.
    Francois Horreard, NanoSIMS Product Manager from CAMECA leads the discussion which will include:

    • making use of 15N-thymidine to label DNA, map and quantify the cell division cycle of single cells

    • answering the immortal (DNA) strand hypothesis by combining 15N DNA labeling, BrdU or IdU marking with pulse-chase experiments.

    • elucidating the origin of cardiomyocytes (from division of pre-existing cardiomyocytes or from stem cells ?) through the use of 15N-thymidine.

    • evidencing the existence of an age mosaicism across multiple scales from tissue, to cell and protein levels.

    The NanoSIMS is used to map tissues of mice fully 15N-labeled and to measure the long-term decay of label during 14N chasing.

    Duration : 43 minutes

  • NS webinars isotopics
    Biological applications of NanoSIMS 50L: using isotopic tracers in lipid studies

    Tuesday, May 26, 2020

    Part 3 of our biology webinar series is dedicated to the use of isotopic tracers in biology and its application to lipid studies.

    Francois Horreard, NanoSIMS Product Manager from CAMECA leads the discussion which will include:

    • making use of isotopes in SIMS analysis & building experiments based on the supply of one or several isotopically labeled compound(s) to living cells, tissues, plants or animals.

    • reviewing various sample preparation & analysis approach

    • taking advantage of the unique sensitivity/spatial resolution capabilities of the NanoSIMS 50L to map the distribution and fluxes of such isotopes with intracellular resolution (50 nanometers)

    • and more!

    Duration : 39 minutes

  • Cell Biology
    Cellular Biology Applications of NanoSIMS 50L Part 2

    Tuesday, March 10, 2020

    Part 2 is dedicated to Nano-object imaging and molecular imaging with elemental tracers.
    After a short introduction to the specifics of biological sample preparation, a selection of recent studies using NanoSIMS are reviewed, including:

    • Food-grade TiO2 nanoparticle toxicology study

    • Silver nanoparticle interactions with green Alga

    • Multiplexed ion beam imaging (MIBI) of human breast tumors

    • and more!

    Presenter: François Horreard, CAMECA

    Duration: 27 minutes

  • Cellular Biology Application
    Cellular Biology Applications of NanoSIMS 50L Part 1

    Monday, March 9, 2020

    Part 1 of the webinar "NanoSIMS 50L Applications in Cellular Biology" is dedicated to trace elements and metal-based drugs in cells.
    After a short introduction to the specifics of biological sample preparation, a selection of recent studies using NanoSIMS are reviewed, including:

    • Detection of copper in retina photoreceptors of zebrafish

    • Subcellular localisation of metals in Arabidopsis thaliana leaf cells

    • Mapping of cisplatin in ovarian cancer cultured cells

    • Anticancer gold-based drug development

    • and more!

    Presenter: François Horreard, CAMECA

    Duration : 31 minutes

  • Light Elements
    Optimization of the Detection Limits for Light Elements (H,C,O) using Dynamic SIMS

    Wednesday, January 22, 2020

    This webinar focuses on the optimization of the detection limits for light elements (hydrogen, carbon, and oxygen atmospheric species) in silicon-based materials using dynamic SIMS. Information on H, C an d O low-level impurities introduced during processing and/or ageing is of major importance for a better understanding of semiconductor device lifetime and failure modes. Dynamic SIMS plays an important role in evaluating the concentration of impurities in solid samples because of its high sensitivity and depth profiling capabilities with high depth resolution and throughput, and the IMS 7f-Auto is designed to achieve optimized detection limits for light elements. Data presented in this webinar shows that significant improvement of H, C, and O detection limits in Si is achieved using the pre-sputtering method. Results obtained using multiple sample holders and the automated storage chamber are also presented.

    Duration : 25 minutes

  • SIMS Nuclear
    Dynamic SIMS for Characterization of Nuclear Materials

    Tuesday, January 14, 2020

    In order to ensure longevity of structural nuclear materials and to improve knowledge on the mechanisms that occur during reactor accidents, it is of great importance to better understand diffusion and migration mechanisms of fission products in nuclear materials and fuel. To that purpose, SIMS analyses are performed on different materials after ion implantation with natural isotopes of high fission yield species (such as molybdenum, helium, xenon, iodine). Application of dynamic SIMS to the characterization of nuclear particles collected in nuclear facilities for safeguards purposes is also discussed.

    Duration : 30 minutes

  • SIMS Principles
    The Principles of Dynamic SIMS

    Monday, January 6, 2020

    This free on-demand webinar is an excellent introduction to the dynamic SIMS technique. Different practical aspects are discussed:

    • Choice of primary and secondary beam species

    • Relative sensitivity for various elements

    • Matrix effects

    • Depth resolution & Lateral resolution

    • Depth profiling (choice of impact energy & sputtering rate, calibration method)

    • Scanning ion imaging mode

    A summary of dynamic SIMS main analytical characteristics is provided at the end.

    Duration : 20 minutes