April 2019

 
SIMS imaging in plant root
SIMS imaging of symbiotic bacteria in plant root

Soybean leaves convert carbon dioxide into sugars by photosynthesis, and transport those sugars to the roots. Specialized bacteria colonize the roots and form nodules, a safe place where the bacteria can live and exchange their nitrogen for sugars from the plant. This sample was taken from a soybean root nodule formed during the symbiosis between nitrogen-fixing bacteria and soybean root cells. The nodules were fixed, embedded in resin, and 500 nm thin sections were cut and mounted on a cover glass for analysis by IMS 7f-GEO. SIMS scanning ion images of 12C14N- on two different areas of a cross section of soybean root show root structure and symbiotic bacteria.
Field of view: 100 x 100 μm2.
Lateral resolution: ~1 μm.

Courtesy of Clive Jones and David Fike, Earth and Planetary Sciences, WUSTL, in collaboration with Christopher Topp and Keith Duncan, Danforth Plant Science Center, USA.